software package visia imaging Search Results


imaging d-sight her2 fish analysis module  (Visia Imaging)
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Visia Imaging imaging d-sight her2 fish analysis module
Characteristics tested tissue specimens of patients with invasive breast cancer with Leica <t> HER2 </t> <t> FISH. </t>
Imaging D Sight Her2 Fish Analysis Module, supplied by Visia Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging d-sight her2 fish analysis module - by Bioz Stars, 2026-04
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Characteristics tested tissue specimens of patients with invasive breast cancer with Leica HER2 FISH.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Characteristics tested tissue specimens of patients with invasive breast cancer with Leica HER2 FISH.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Staining

“Pictures”: at least 4 areas of interest are selected for analysis and pictures are taken automatically. Captured images from different focus planes are combined generating a picture with all signals clearly detectable after removal of unspecific background. “Objectives and tools”: automated nucleus selection and spot counting of the red ( HER2 ) and the green (chromosoom 17 = CEP17 ) signal is evaluated and corrected by the observer if needed. “Analysis”: supervised-automated scoring is performed on at least two pictures and the ratio between HER2 and CEP17 was generated.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: “Pictures”: at least 4 areas of interest are selected for analysis and pictures are taken automatically. Captured images from different focus planes are combined generating a picture with all signals clearly detectable after removal of unspecific background. “Objectives and tools”: automated nucleus selection and spot counting of the red ( HER2 ) and the green (chromosoom 17 = CEP17 ) signal is evaluated and corrected by the observer if needed. “Analysis”: supervised-automated scoring is performed on at least two pictures and the ratio between HER2 and CEP17 was generated.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Selection, Generated

Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in 20 full-sized slides of tissue specimens used for TMA blocks of invasive breast cancer.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in 20 full-sized slides of tissue specimens used for TMA blocks of invasive breast cancer.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in tissue micro arrays (TMAs) including 328 invasive breast cancer tissue specimens.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in tissue micro arrays (TMAs) including 328 invasive breast cancer tissue specimens.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Representative pictures of HER2 non-amplified invasive breast cancer specimens are shown in A) with a HER2 (red signals) to chromosoom 17 (= CEP17 ; green signals) ratio <2 and C) with supervised-automated nuclei and spot detection. Examples of HER2 amplified invasive breast cancer specimens are depicted in B) with a HER2 / CEP17 ratio >2 and D) with supervised-automated nucleus and spot detection. DAPI counterstaining and original magnification with 100x objective.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Representative pictures of HER2 non-amplified invasive breast cancer specimens are shown in A) with a HER2 (red signals) to chromosoom 17 (= CEP17 ; green signals) ratio <2 and C) with supervised-automated nuclei and spot detection. Examples of HER2 amplified invasive breast cancer specimens are depicted in B) with a HER2 / CEP17 ratio >2 and D) with supervised-automated nucleus and spot detection. DAPI counterstaining and original magnification with 100x objective.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Comparisons of Leica HER2 FISH and DAKO HER2 IHC of 50 consecutively collected invasive breast cancer tissue specimens (resection / biopsy) from routine practice.

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of Leica HER2 FISH and DAKO HER2 IHC of 50 consecutively collected invasive breast cancer tissue specimens (resection / biopsy) from routine practice.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification